Photo-irradiation improved functional preservation of the isolated rat heart

✍ Scribed by Zhu, Qingyan; Yu, Wei; Yang, Xiaoping; Hicks, George L.; Lanzafame, Raymond J.; Wang, Tingchung

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 172 KB
• Volume: 20
• Category: Article
• ISSN: 0196-8092
• DOI: 10.1002/(sici)1096-9101(1997)20:3<332::aid-lsm12>3.0.co;2-f

✦ Synopsis

Background and Objective: Photo-irradiation causes a variety of effects in different cells and tissues. We hypothesized that photoirradiation may improve cardiac preservation based on these observations. Study Design/Materials and Methods: For pre-storage treatment (Pre), the heart in an anesthetized open-chest rat was irradiated using an Argon-dye laser with a wavelength of 660 nm at a fluence of 16.8 J/cm 2 or sham-operated. The heart was excised, perfused with Krebs-Henseleit Buffer, cardioplegically arrested, and stored by immersion at 0°C for 18 hrs. Functional recovery was evaluated by working reperfusion for 30 min. For post-storage treatment (Post), the isolated hearts were stored for 18 hrs at 0°C; laser irradiation at a fluence of 36 J/cm 2 was administered during working reperfusion. Hearts which did not receive irradiation during reperfusion served as control. Furthermore, isolated cardiomyocytes were used to study laser effect on cellular ATP content, catalase activity, and nitric oxide (NO) release. Results: Both Pre and Post groups showed significant improvement in recovery of aortic flow, cardiac output, and work compared to the corresponding control groups (P<0.05). Combined Pre/Post laser treatment did not improve function. Investigation using isolated rat cardiomyocytes found that both end-storage ATP and end-reperfusion catalase activity in the laser-treated group were significantly higher than those in the untreated cells (P<0.05). NO release increased by 15% in the laser group after 18 hrs of 37°C incubation.

Conclusion: