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Photo-Induced Protein-RNA Cross-Linking in Mammalian 40-S Ribosomal Subunits

✍ Scribed by Anne-Marie REBOUD; Monique BUISSON; Marie-Jeanne MARION; Jean-Paul REBOUD


Book ID
115116911
Publisher
John Wiley and Sons
Year
1978
Tongue
English
Weight
780 KB
Volume
90
Category
Article
ISSN
1432-1327

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The use of sym-triazine trichloride in R
✍ Oste, Christian ;Brimacombe, Richard πŸ“‚ Article πŸ“… 1979 πŸ› Springer 🌐 English βš– 578 KB

The reagent sym-triazine trichloride is used as a bifunctional reagent to generate RNA-protein cross-links within intact ribosomal subunits from E. coli. The reaction takes place in a stepwise manner, involving substitution of one chlorine atom at 12 degrees and pH 8, and substitution of the second