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Phosphorylation of C8 and C9 Subunits of the Multicatalytic Proteinase by Casein Kinase II and Identification of the C8 Phosphorylation Sites by Direct Mutagenesis †

✍ Scribed by Castaño, José G.; Mahillo, Esther; Arizti, Paz; Arribas, Joaquín


Book ID
126216263
Publisher
American Chemical Society
Year
1996
Tongue
English
Weight
525 KB
Volume
35
Category
Article
ISSN
0006-2960

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A commercial soybean isolate was phosphorylated using casein kinase II puri®ed from the yeast Yarrowia lipolytica. Both major reserve proteins, b-conglycinin and glycinin, were phosphorylated in a sequential way. The soybean isolate incorporated up to 0.7 mol phosphate per mole in 2 h. It was found