Phosphorus metabolites and the distribution of cell cycle phase of RIF-1 tumors in response to 14 Gy irradiation
✍ Scribed by Anthony W. Majors; Thian C. Ng; Ipatia M. Karalis; Mark G. Edinger; Raymond R. Tubbs; Patrick D. Higgins; Kyo H. Shin
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 343 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0740-3194
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✦ Synopsis
Abstract
Simultaneous measurements of DNA cell phase cycle distributions and in vivo ^31^P NMR spectroscopy were performed on 40 RIF‐1 murine tumors irradiated with 14 Gy of X‐radiation. Diploid and tetraploid tumor populations were observed. The cells blocked in G~2~/M phase were measured as a function of the ratios of tetraploid cell number in G~2~/ M phase versus total cell population measured. The G~2~/M population reached a maximum at 32 h post irradiation, dropping to control values by 72 h, while the ratio of inorganic phosphate to β‐nucleotide triphosphate dropped significantly at 32 h and remained significantly lower than control up to 72 h post irradiation. Measurements of PME, PDE, PCr, and pH showed no significant variations at any time point. No significant change in host cell population could be observed. Since the measured G~2~/M population never increased to more than 3% of the total cell population, the change observed in the ^31^P NMR spectra were not simply the result of possible differences in NMR profiles of the different cell phase populations but were more likely due to a change in the metabolic characteristics or environment of a majority of the Cells. © 1990 Academic Press, Inc.