Phosphorus J-coupling constants of ATP in human brain

✍ Scribed by Wulf-Ingo Jung; Andreas Staubert; Stefan Widmaier; Thomas Hoess; Michael Bunse; Franz van Erckelens; Günther Dietze; Otto Lutz

• Book ID: 102531808
• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 321 KB
• Volume: 37
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910370525

✦ Synopsis

Abstract

Proton decoupled ^31^P NMR spectroscopy of the occipital brain of healthy volunteers was performed with a 1.5 T whole‐body imager. By use of two‐dimensional chemical‐shift imaging in combination with slice‐selective excitation well resolved localized spectra (38 ml) were obtained within 34 min from which the homonuclear ^31^P‐^31^P J‐coupling constants of ATP could be determined: J~αβ~ = 16.1 Hz ± 0.2 Hz and J~αβ~ = 16.3 Hz ± 0.1 Hz (mean ± SEM, n = 14). Both, the J‐coupling constants and the chemical‐shift difference between α‐ and β‐ATP (δ~αβ~ = 8.61 ppm ± 0.01 ppm) were used to calculate the concentration of intracellular free magnesium. The concentrations are 0.39 m__M__ ± 0.09 m__M__ by using the average of both coupling constants of each spectrum, which is in fair agreement with 0.32 m__M__ ± 0.01 m__M__ obtained from the chemical shift of α and β phosphate resonances, which is the more accurate result.