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Phosphoribosylpyrophosphate synthetase of Bacillus subtilis. Cloning, characterization and chromosomal mapping of the prs gene

โœ Scribed by Nilsson, Dan; Hove-Jensen, Bjarne


Book ID
122897729
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
846 KB
Volume
53
Category
Article
ISSN
0378-1119

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Cloning and characterization of the prs
โœ Hove-Jensen, Biarne ๐Ÿ“‚ Article ๐Ÿ“… 1985 ๐Ÿ› Springer ๐ŸŒ English โš– 865 KB

The gene, prs, encoding phosphoribosylpyrophosphate (PRPP) synthetase of Escherichia coli was isolated from a library of E. coli genes cloned in the bacteriophage 2D69 vector. A strain with a temperature-lethal defect in PRPP synthetase, (prs-2), was used as the host and cloning was performed by lys