For the development of valid algorithms for matching protein spots between two-dimensional gels, it is essential that one has an understanding of the relative roles of the many sources of variability affecting the location of spots. We first consider the contribution of observers to the measurement
Phosphoproteome Profiling Using a Fluorescent Phosphosensor Dye in Two-Dimensional Polyacrylamide Gel Electrophoresis
โ Scribed by Mieko Otani; Taizo Taniguchi; Akiko Sakai; Jouji Seta; Keiichi Kadoyama; Tooru Nakamura-Hirota; Shogo Matsuyama; Keiji Sano; Masaoki Takano
- Book ID
- 107565839
- Publisher
- Springer-Verlag
- Year
- 2011
- Tongue
- English
- Weight
- 621 KB
- Volume
- 164
- Category
- Article
- ISSN
- 0273-2289
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The characteristics of protein detection and quantitation with SYPRO Ruby protein gel stain in one- and two-dimensional polyacrylamide gels were evaluated. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analyses of three different purified recombinant proteins showed that the l
## Standardization of protein position in silver-stained two-dimensional poly acrylamide gel electrophoresis Methods for standardization of silver-"stained" protein position in two-dimensional polyacrylamide gel electrophoresis are presented for their application to simultaneous multiple gel syste