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Phospholipid synthesis-dependent activity of aminopeptidase N in intact cells of Escherichia coli

✍ Scribed by Andrée Lazdunski; Maryse Murgier; Claude Lazdunski


Book ID
118918073
Publisher
Elsevier Science
Year
1979
Tongue
English
Weight
909 KB
Volume
128
Category
Article
ISSN
0022-2836

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The pepN gene, that encodes aminopeptidase N in Escherichia coli, has been cloned into the multicopy plasmid pBR322. Expression of the cloned pepN gene results in overproduction of the enzyme. The restriction map of the 6.7 Kb insert was established and the gene was further localized by analysis of