Phospholipid headgroup dynamics in DOPG-d5-cytochrome c complexes as revealed by 2H and 31P NMR: The effects of a peripheral protein on collective lipid fluctuations
✍ Scribed by Teresa J.T Pinheiro; Melinda J Duer; Anthony Watts
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 931 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0926-2040
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✦ Synopsis
The dynamics of the glycerol headgroup of dioleoylphosphatidylglycerol (DOPG) in hydrated bilayers were studied by 'H and 3'P NMR spectroscopy, and the effects of binding a peripheral protein, cytochrome c, were evaluated. The fast headgroup segmental motions (TV, 10-'"-lO-I3 s> of DOPG in fully hydrated bilayers were not affected upon binding of cytochrome c, as evaluated by the spin-lattice CT,) relaxation of deuterons in the DOPG glycerol headgroup. In contrast, the spin-spin (T&) relaxation is strongly affected, indicating that slow cooperative bilayer motions (TV,, 10-3-10-6 s) are enhanced upon the interaction with cytochrome c. *H and 3'P NMR spectral lineshape analysis reveal details of the nature of these motions. The importance of these effects are discussed in terms of a possible mechanism for modulating membrane-associated processes.