✦ LIBER ✦
Phospholipase activity of sea urchin sperm: Its possible involvement in membrane fusion
✍ Scribed by Conway, A. F. ;Metz, C. B.
- Publisher
- John Wiley and Sons
- Year
- 1976
- Tongue
- English
- Weight
- 840 KB
- Volume
- 198
- Category
- Article
- ISSN
- 0022-104X
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✦ Synopsis
Abstract
Phospholipase activity of egg‐water treated Arbacia punctulata and Lytechinus variegatus sperm was shown to result from the sequential action of phospholipase A and lysophospholipase. A transient burst of phospholipase A activity followed induction of the acrosome reaction with egg water. The time of appearance suggested an acrosomal localization of the enzyme. The peak activity of phospholipase A correlated with initiation of sperm‐egg fusion, suggesting a role for sea urchin sperm phospholipase A in membrane fusion and/or egg activation during fertilization.