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Phospholipase A2 assay using an intramolecularly quenched pyrene-labeled phospholipid analog as a substrate

✍ Scribed by Tom Thuren; Jorma A. Virtanen; Pentti J. Somerharju; Paavo K.J. Kinnunen


Book ID
107714557
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
604 KB
Volume
170
Category
Article
ISSN
0003-2697

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Intramolecularly Quenched BODIPY-Labeled
✍ H.Stewart Hendrickson; Elizabeth K. Hendrickson; Iain D. Johnson; Steven A. Farb πŸ“‚ Article πŸ“… 1999 πŸ› Elsevier Science 🌐 English βš– 200 KB

Phospholipase substrate analogs containing both a fluorescent BODIPY group and a quenching 2,4-dinitrophenyl (DNP) group were synthesized. They showed little fluorescence, but upon hydrolysis became fluorescent as the quenching group was removed. Two substrates were phosphatidylethanolamine analogs