Phosphoinositide 3-kinase inhibition enables retinoic acid-induced neurogenesis in monolayer culture of embryonic stem cells
✍ Scribed by Hana Kotasová; Iva Veselá; Jan Kučera; Zbyněk Houdek; Jiřina Procházková; Milena Králičková; Jiří Pacherník
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 283 KB
- Volume
- 113
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
Retinoic acid (RA) is able to induce the differentiation of embryonic stem cells into neuronal lineages. The mechanism of this effect is unknown but it has been evidenced to be dependent on the formation of floating spheroids called embryoid bodies. Results presented here show that the inhibition of phosphoinositide 3‐kinase signaling pre‐determines mouse embryonic stem cells to RA induced neurogenesis in monolayer culture with no need of embryoid bodies formation. J. Cell. Biochem. 113: 563–570, 2012. © 2011 Wiley Periodicals, Inc.