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Phosphohistidine Analysis Using Reversed-Phase Thin-Layer Chromatography

✍ Scribed by Paul G. Besant; Michael V. Lasker; Cuong D. Bui; Christoph W. Turck

Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
172 KB
Volume
282
Category
Article
ISSN
0003-2697

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✦ Synopsis

Proteins phosphorylated on histidine residues which are common to bacteria as part of the two-component signaling mechanism (1) are also becoming of increasing interest in eukaryotic signaling pathways (2). Many of these proteins are phosphorylated transiently on histidine, with the phosphate moiety subsequently

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## Abstract The chromatographic behavior of some hydrocarbons and some of their nitro derivates after preconditioning with solvent vapors has been investigated. After preloading with cyclohexane or hexane an improved separation of some sample pairs is observed. Furthermore, inversion of R~f~ values