Phosphoglucomutase-1 subtyping of human bloodstains on ultrathin-layer polyacrylamide gels

✍ Scribed by Bruce Budowle

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 311 KB
• Volume: 5
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150050309

✦ Synopsis

Phosphoglucomutase-1 subtyping of human bloodstains on ultrathin-layer polyacrylamide gels

A method is described for obtaining nondistorted, reproducible phosphoglucomutase-1 subtyping patterns from fresh human bloodstains by ultrathin-layer polyacrylamide gel isoelectric focusing. Electrofocusing of phosphoglucomutase on 0.2 mm thick gels took 52 and 80 min on the Cold Focus Apparatus and Ultrophor, respectively, when the distance separating the electrodes was 8.0 cm, whereas a 9.5 cm distance between electrode wicks required 95 min on the Multiphor to complete isoelectric focusing. The gels with 8.0 cm between electrode wicks provided sharper band patterns compared with the gels with 9.5 cm between electrode wicks.