Phosphoenolpyruvate carboxylase: three-dimensional structure and molecular mechanisms
β Scribed by Yasushi Kai; Hiroyoshi Matsumura; Katsura Izui
- Book ID
- 117042540
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 883 KB
- Volume
- 414
- Category
- Article
- ISSN
- 0003-9861
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Phosphoenolpyruvate carboxylase (EC 4.1.1.31) was purified 43-fold from Amaranthus viridis leaves by using a combination of ammonium-sulphate fractionation, chromatography on O-(diethylaminoethyl)-cellulose and hydroxylapatite, and filtration through Sepharose 6B. The purified enzyme had a specific
A gene (SCPEPCD1) encoding phosphoenolpyruvate carboxylase (PEPC) was isolated from the C-4 monocot sugarcane (Saccharum hybrid var. H32-8560). SCPEPCD1 is ca. 6800 bp long, with 10 exons. The entire gene sequence from -1561 to 262 bp downstream of the putative poly(A) addition signal is reported. A