Phosphodiesters in saponified extracts of human breast and colon tumors using 31P magnetic resonance spectroscopy

✍ Scribed by Thomas E. Merchant; Dainius Characiejus; John N. Kasimos; Willem Den Otter; Louis W. Gierke; Thomas Glonek

• Publisher: John Wiley and Sons
• Year: 1992
• Tongue: English
• Weight: 576 KB
• Volume: 26
• Category: Article
• ISSN: 0740-3194
• DOI: 10.1002/mrm.1910260114

✦ Synopsis

Abstract

Saponified phospholipid extracts of malignant and normal human breast and colon surgical tissue specimens (n = 45) generate characteristic phosphodiester profiles using ^31^P magnetic resonance (MR) spectroscopy. The resultant ^31^P MR spectroscopic profiles of the analyzed tissues are used to differentiate malignant from normal. The appearance of an uncharacterized resonance at 0.29 δ in the malignant tissue spectra (50% of breast and 75% of colon specimens) is the most notable qualitative finding. Quantitatively, malignant colon tissues differ from normal colon tissues with depressed levels of phosphatidylserine and elevated levels of glycerol 3‐phosphorylglycerol and an index measuring the summation of phospholipid polar head group residues with free hydroxyl groups. Malignant breast tissues have significantly elevated levels of glycerol 3‐phosphorylethanolamine and significantly depressed levels of glycerol 3‐phosphorylcholine compared to normal breast tissues, reflecting a perturbation in the balance of lipid residues that are the respective breakdown products of phosphatidylethanolamine and phosphatidylcholine. The concentration of the polar head group residues is compared to ^31^P MR spectroscopic profiles of colon and breast tissue phospholipids, in order to demonstrate the quantitative nature of the technique employed.