Phosphatidylserine/Cholesterol Bilayers Supported on a Polycation/Alkylthiol Layer Pair
✍ Scribed by Liqin Zhang; Carrie A. Booth; Pieter Stroeve
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 133 KB
- Volume
- 228
- Category
- Article
- ISSN
- 0021-9797
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✦ Synopsis
1-Stearoyl-2-oleoyl phosphatidylserine (SOPS)/cholesterol bilayers, supported on a polycation/alkylthiol layer pair on a gold surface, were investigated by surface plasmon resonance (SPR) and fluorescence recovery after photobleaching. The substrate was formed by electrostatic adsorbance of a hydrated poly(diallyldimethylammonium chloride) (PDDA) layer on the negatively charged surface of a self-assembled monolayer of 11-mercaptoundecanoic acid (MUA) on gold. Lipid membranes with different SOPS/cholesterol compositions were deposited on the PDDA/MUA layer pair by vesicle fusion. When the cholesterol content was below 20%, single bilayers were deposited. Fluorescence recovery after the bleaching experiments revealed that the SOPS/cholesterol bilayers were mobile at room temperature; lateral diffusion coefficients of a fluorescence probe were approximately 1x10(-9) cm(2)/s. The kinetics of the addition of the ion-channel-forming peptide gramicidin to the supported bilayers was detected by SPR. Copyright 2000 Academic Press.