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Phosphatidylinositol 4,5-bisphosphate competitively inhibits phorbol ester binding to protein kinase C

โœ Scribed by Chauhan, Abha; Chauhan, Ved P. S.; Deshmukh, D. S.; Brockerhoff, Hans


Book ID
126793170
Publisher
American Chemical Society
Year
1989
Tongue
English
Weight
624 KB
Volume
28
Category
Article
ISSN
0006-2960

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Microsomes were prepared from cultured neonatal rat cardiomyocytes. Incubation of microsomes in buffer containing 5 microM CaCl2, 5 mM cholate and 100 nM [3H-]Phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5) P2) resulted in the formation of [3H-]InsP3. GTP-gamma-S (125 microM) stimulated the produ