๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Phosphate regulation in Pseudomonas aeruginosa: Cloning of the alkaline phosphatase gene and identification of phoB- and phoR-like genes

โœ Scribed by Filloux, A. ;Bally, M. ;Soscia, C. ;Murgier, M. ;Lazdunski, A.


Publisher
Springer
Year
1988
Tongue
English
Weight
487 KB
Volume
212
Category
Article
ISSN
0026-8925

No coin nor oath required. For personal study only.

โœฆ Synopsis


In Pseudomonas aeruginosa, phosphate limitation results in the synthesis of several protein species. We report the cloning of the P. aeruginosa alkaline phosphatase structural gene, phoA, and we show that this gene is regulated normally in Escherichia coli. We have also identified and cloned two P. aeruginosa genes which can complement phoB and phoR mutations in E. coli. This suggests that a pho regulon system similar to that in E. coli may exist in P. aeruginosa, using at least two similar regulatory factors.


๐Ÿ“œ SIMILAR VOLUMES


Mutations affecting regulation of the an
โœ Itoh, Yoshifumi ;Matsumoto, Hideki ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 965 KB

The nucleotide sequence required for a fully functional promoter and operator of the Pseudomonas aeruginosa argF gene (argFpo), the arginine-repressible gene for anabolic ornithine carbamoyltransferase, was defined within a 160 bp region. The streptomycin (Sm) resistance genes strAB of plasmid RSF10

Cloning and nucleotide sequence of the g
โœ Hoshino, Toshimitsu ;Kose, Kumiko ;Uratani, Yoshihiko ๐Ÿ“‚ Article ๐Ÿ“… 1990 ๐Ÿ› Springer ๐ŸŒ English โš– 728 KB

The gene braB, encoding the Na+-coupled carrier for branched-chain amino acids in Pseudomonas aerugin- osa PAO, was cloned on cosmid pMMB34. The cosmid clones carrying the braB gene were identified as those that restored growth at low leucine concentration and Na+-dependent leucine transport activit

Expression of histone and alkaline phosp
โœ Dr. M. H. Zheng; S. Wysocki; J. M. Papadimitriou; D. Wood ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 555 KB

The fluorescent Dye H33342 (H342) is a bis-benzimidazole used for intravital fluorescent staining. In this report, we found that H342 completely abolished histone 2a mRNA but had no effect on alkaline phosphatase gene expression and protein synthesis in UMR 106-01 rat osteoblast-like cells. The comp