Phloem transport of14C-labelled assimilates inRicinus
โ Scribed by Shelagh M. Hall; D. A. Baker; J. A. Milburn
- Book ID
- 104750501
- Publisher
- Springer-Verlag
- Year
- 1971
- Tongue
- English
- Weight
- 394 KB
- Volume
- 100
- Category
- Article
- ISSN
- 0032-0935
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โฆ Synopsis
Exudate can be obtained from incisions made in the bark of the stem of actively growing Ricinus plants, ltC-labelled assimilates from a fed leaf are rapidly detected in the exudate. This movement was both acropetal and basipetal from the fed leaf, at rates of over 100 cm h -1. Estimated rates within intact plants were 80-84 cm h -1.
In contrast with xylem sap obtained from the same plant, the exudate obtained had an alkaline pH (8.2), a high dry matter content (10-12.5 % ), high sugar content (8-10%) which was predominantly sucrose; high potassium content (60-80 raM) and low calcium content (0.5-1.0 ml~I).
It is concluded, on the basis of the present evidence, that the exudate is a true sample of the sieve tube sap undergoing translocation.
๐ SIMILAR VOLUMES
After the laminae of leaves of intact plants had been exposed to ~4C02 the translocation of 14C-labelled assimilates across the petioles starts very quickly: 6 to 1O rain later ~4C-activity could be detected in the basal part of the petioles. The way of distribution within the plant seems to be infl
It is widely accepted that the floral stimulus produced in leaves is carried to the shoot apex passively in the phloem with the assimilate s~ream. Three kinds of evidence presented here suggest that the floral stimulus moves independently of the assimilates. Simultaneous determination of the veloci
The osmotic characteristics of phloem-sap exudation were examined in soil-grown and watercultured plants of Ricinus communis L. Prolonged exudation occurred from bark incisions in water-cultured plants. Fresh incisions caused large alterations in solute flux, but phloem-sap solute potential ฮจs chang
Phloem exudation from Ricinus has been examined in plants subjected to changes in water balance induced by a number of means. The results have provided a clear demonstration that the phloem system can operate osmotically. When the availability of water in the xylem is reduced by withholding water, t
Translocation of 14C-labelled assimilates down the petioles was studied in intact plants of Pelargonium zonale (L.) L'H6rit ex Ait. The central bundle of the petiole was dissected out and treated with solutions of various inhibitors. Whereas cytochalasin B had no effect on l~C-translocation, a disti