Phenotypically variant adrenal tumor cell cultures with biochemical lesions in the ACTH-stimulated steroidogenic pathway
✍ Scribed by Bernard P. Schimmer
- Book ID
- 102878846
- Publisher
- John Wiley and Sons
- Year
- 1969
- Tongue
- English
- Weight
- 546 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Four clonal adrenal tumor cell lines which exhibit biochemical lesions i n the ACTH-stimulated steroidogenic pathway have been isolated. Two of these cell lines, designated Y-6 and 0'33, appear to contain their lesions at points proximal to cyclic AMP formation i n the ACTH-stimulated steroidogenic pathway. Growth of Y-6 and OS3 as tumors in isogenic mice results i n a restoration of ACTH seniitivity i n both cell lines by mechanisms which do no: appear to involve selection or fulfillment of specific nutritional requirements. Growth of Y-6 and OS3 as tumors in heterogenic mice results in restoration of AClH sensitivity in Y-6 but not in 053, suggesting that the biochemical lesions in these cell lines are at different loci. Two other cell lines, designated OS1 and OS4, possess biochemical lesions i n the steroidogenic pathway beyond the formation of cyclic AMP and before the formation of pregnenolone. Growth of OS1 and OS4 as tumors in isogenic mice results in the repair of the biochemical lesions in these cells distal to cyclic AMP formation in the ACTH-stimulated steroidogenic pathway. The four cell lines described are potentially useful in elucidating the mechanism of action of ACTH i n adrenal cells as well as in determining the factors required for maintaining differentiated function in cultured cells.