Phenotypic variation in plants regenerated from protoplasts: The potato system
β Scribed by Dennis L. Bidney; James F. Shepard
- Publisher
- John Wiley and Sons
- Year
- 1981
- Tongue
- English
- Weight
- 548 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0006-3592
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β¦ Synopsis
Abstract
Regeneration of whole plants from isolated protoplasts (plant cells devoid of cell walls) provides a novel capability that is potentially useful for crop improvement efforts. Such a regeneration capacity has been developed for the commercial potato cultivar βrusset Burbank,β currently the most popular cultivar in production. Due to fertility problems of this cultivar, the improvement of βrusset Burbankβ by classical breeding procedures has been limited. Examination of a large population of protoplastβderived clones has revealed the variation for a number of traits can be observed. Variation observed under laboratory conditions and in field trials includes changes in plant morphology and tuberβsetting characteristics, as well as alterations in response to environmental and pathogen stress. A brief description of the cloning process and the potential for application of cloning technology in crop plant improvement will be presented.
π SIMILAR VOLUMES
Protoplasts were produced from 7-day-old hypocotyls of two cultivated sunflower genotypes and three wild sunflowers. When included in agarose droplets and cultured in TL medium supplemented with 0.1 mg/l 2,4-dichlorophenoxyacetic acid, the protoplasts gave rise to loose colonies and to "embryoids".
Enzymatic digestion of newly expanded leaves of glasshouse-grown seedlings of passionfruit released protoplasts which exhibited highest division frequency (38.6%) when plated at a density of 1.5Γ10(5) ppts ml(-1) in agarose-solidified droplets of KM8P medium containing the antibiotic cefotaxime (250