Phenotypic changes associated with chemically induced differentiation of a hairy cell leukemia cell line

✍ Scribed by W. Craig Hooper; Rolf F. Barth; Jun Minowada

• Book ID: 102700433
• Publisher: John Wiley and Sons
• Year: 1987
• Tongue: English
• Weight: 991 KB
• Volume: 24
• Category: Article
• ISSN: 0361-8609
• DOI: 10.1002/ajh.2830240410

✦ Synopsis

In v i m chemically induced differentiation of the JOK-1 cell line, derived from a patient with hairy cell leukemia, has been studied. Non-induced JOK-1 cells expressed sIgM, B-1, CALLA, Ia, and C3 receptors. Following exposure to 2% DMSO, or 100 n g / d 12-0-tetradecanoyl-phorbol-13-acetate (TPA), the cells underwent a phenotypic change from lymphoblastoid to monocytoid. In the presence of TPA, they became adherent and developed dendritic processes. Associated with these changes was a decrease in sIgM and CALLA and variable expression of C3 receptors. No monocyte/ macrophage-associated antigens were detected. These results suggest that the JOK-1 cell line may have a limited ability to differentiate but is not mature enough to complete the differentiation process.