Phenotype and gene expression pattern of osteoblast-like cells cultured on polystyrene and hydroxyapatite with pre-adsorbed type-I collagen
✍ Scribed by Nobutaka Hanagata; Taro Takemura; Akira Monkawa; Toshiyuki Ikoma; Junzo Tanaka
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 434 KB
- Volume
- 83A
- Category
- Article
- ISSN
- 1549-3296
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✦ Synopsis
Abstract
Hydroxyapatite and type‐I collagen are major components of bone matrix. We compared phenotype and gene expression pattern of osteoblast‐like cells cultured on HAp and HAp with pre‐adsorbed type‐I collagen from neutral solutions (HAp/NCs) with those of tissue culture grade polystyrene (TCPS) and TCPS with the collagen (TCPS/NCs). In initial cell attachment, the cells tensely spread on TCPS, while loosely spread on TCPS/NCs, HAp, and HAp/NCs. The levels of expressed integrin α~2~ and α~5~ subunits were not significantly different among any surfaces. Although the cells on HAp/NCs directly attached with the same collagen molecules as TCPS/NCs, mineralization was observed in only TCPS/NCs. The basal substrates (TCPS and HAp) greatly influenced osteoblast maturation even in the surfaces with pre‐adsorbed collagen, since mineralization was induced by TCPS, but not by HAp. Gene expression pattern analyzed with DNA microarray also supported greater influence of basal substrates than pre‐adsorbed collagen. In addition, comprehensive gene expression analyses revealed that HAp and HAp/NCs specifically up‐regulated Ly6a and Tmem37 genes, while down‐regulated Ifitm5 gene. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007