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Phase diagram determination for phospholipid/sterol membranes using deuterium NMR

✍ Scribed by Ya-Wei Hsueh; Martin Zuckermann; Jenifer Thewalt


Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
210 KB
Volume
26A
Category
Article
ISSN
1546-6086

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✦ Synopsis


Deuterium NMR is used to investigate the phase behavior of membranes containing ergosterol and to measure the temperature-ergosterol mole fraction phase diagram. In particular, our approach to analyzing the NMR spectra has allowed us to definitively locate and characterize the region where two liquid crystalline phases coexist. The general methodology for phase boundary determination in phospholipid/sterol membranes is presented.