In order to study the intracellular metabolism of Methotrexate (MTX) and the cytotoxicity of the antifolates, a specific paired-ion HPLC method has been developed which permits the simultaneous determination of DAMPA, MTX, 7-OH-MTX, MTX-G1, MTX-G2 and MTX-G3. Cells were incubated with 3H-MTX. The MT
Pharmacokinetics of methotrexate (MTX) and 7-hydroxymethotrexate (7-OH-MTX) in rats and evidence for the metabolism of MTX to 7-OH-MTX
โ Scribed by Lutz Fahrig; Helmut Brasch; Heiko Iven
- Publisher
- Springer
- Year
- 1989
- Tongue
- English
- Weight
- 444 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0344-5704
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The permeability of 19 compounds in both the Caco-2/TC7 and HT29-MTX models was determined, and the ability of each model to predict intestinal absorption in humans was compared. Similar apparent permeability values (log P app ) were obtained in both models for the majority of compounds tested, and
## Abstract Dehydroepiandrosterone (DHEA) is an important neurosteroid with neuronal protection and memory enhancement functions. 7ฮฑโOH DHEA and 7ฮฒโOH DHEA are the two important metabolites of DHEA in the brain. We have developed an LC/MS method to quantitatively analyze 7ฮฑโOH DHEA and 7ฮฒโOH DHEA.