Pharmacokinetic study of p-coumaric acid in mouse after oral administration of extract of Ananas comosus L. leaves

✍ Scribed by Zhen Meng; Wei Wang; Dong-Ming Xing; Fan Lei; Jia-Qi Lan; Li-Jun Du

• Publisher: John Wiley and Sons
• Year: 2006
• Tongue: English
• Weight: 158 KB
• Volume: 20
• Category: Article
• ISSN: 0269-3879
• DOI: 10.1002/bmc.626

✦ Synopsis

Abstract

Quantification of __p‐__coumaric acid in mouse plasma following oral administration of Ananas comosus L. leaves was achieved by reversed‐phase high‐performance liquid chromatography using a mobile phase of water–acetonitrile (82:18, v/v) and UV detection at 310 nm. The method was linear (determination coefficient, r^2^ = 0.9997) within the tested range (0.04–1.28 µg/mL). Intra‐ and inter‐day precision coefficients of variation and accuracy bias were acceptable (maximal CV value was 4.06% for intra‐day and 4.19% for inter‐day) over the entire range. The recoveries were 90.63, 97.98 and 100.01% for concentrations of 0.04, 0.32 and 1.28 µg/mL, respectively. This is a very rapid, sensitive and economical way to determine __p‐__coumaric acid concentration in mouse plasma after oral administration of A. comosus leaves. The concentration–time curve was fitted to the one‐compartment model. This is the first time that __p‐__coumaric acid extracted from A. comosus leaves was detected by HPLC‐UV method and its pharmacokinetic characteristic was comprehensively studied. Copyright © 2006 John Wiley & Sons, Ltd.