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Phage display screening for peptidic chitinase inhibitors

✍ Scribed by Cordula Petter; Christa Scholz; Helga Wessner; Gerd Hansen; Peter Henklein; Takeshi Watanabe; Wolfgang Höhne

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
420 KB
Volume
21
Category
Article
ISSN
0952-3499

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✦ Synopsis

Abstract

A phage display library with disulfide‐cyclized peptides was screened for peptides binding to chitinases from Serratia marcescens. One of those peptides was found to efficiently inhibit chitinase A and two others were inhibitors of chitinase B. Complete substitutional analysis of all three peptides using cellulose‐bound peptide spot synthesis revealed key interaction positions and allowed optimization of the chitinase B inhibitory peptides towards higher affinity, with inhibitory constants in the lower nanomolar range. Inhibition by all peptides proved to be competitive and highly specific for the chitinase used to select them, as shown with a series of chitinases from different organisms. Copyright © 2008 John Wiley & Sons, Ltd.

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