Phage Display: Methods and Protocols

✍ Scribed by Michael Hust, Theam Soon Lim (eds.)

• Publisher: Humana Press
• Year: 2018
• Tongue: English
• Leaves: 556
• Series: Methods in Molecular Biology 1701
• Edition: 1
• Category: Library

✦ Synopsis

This volume provides comprehensive explanations and detailed examples of different antibody libraries, along with novel approaches for antibody discovery. The chapters in this book are divided into four sections: 1) construction of antibody libraries; 2) selection strategies for antibodies; 3) complementary approaches for antibody selection; and 4) phage display for epitope mapping and biomarker identification. The chapters also provide a list of antibody phage display technologies and applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Phage Display: Methods and Protocols will provide technical assistance to new start-ups venturing into the field of antibody phage display.

This volume will also aid in stirring interest and ideas among researchers in this ever-expanding subject.

✦ Table of Contents

Front Matter ....Pages i-xiv
Front Matter ....Pages 1-1
Construction of Human Immune and Naive scFv Libraries (Jonas Kügler, Florian Tomszak, André Frenzel, Michael Hust)....Pages 3-24
Construction of Naive and Immune Human Fab Phage-Display Library (Noorsharmimi Omar, Theam Soon Lim)....Pages 25-44
Construction of Synthetic Antibody Phage-Display Libraries (Johan Nilvebrant, Sachdev S. Sidhu)....Pages 45-60
Modular Construction of Large Non-Immune Human Antibody Phage-Display Libraries from Variable Heavy and Light Chain Gene Cassettes (Nam-Kyung Lee, Scott Bidlingmaier, Yang Su, Bin Liu)....Pages 61-82
Construction of Macaque Immune-Libraries (Arnaud Avril, Sebastian Miethe, Michael Hust, Thibaut Pelat)....Pages 83-112
Construction of Bovine Immunoglobulin Libraries in the Single-Chain Fragment Variable (scFv) Format (Ulrike S. Diesterbeck)....Pages 113-131
Construction of Rabbit Immune Antibody Libraries (Thi Thu Ha Nguyen, Jong Seo Lee, Hyunbo Shim)....Pages 133-146
Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries (Julius Grzeschik, Doreen Könning, Steffen C. Hinz, Simon Krah, Christian Schröter, Martin Empting et al.)....Pages 147-167
Construction of High-Quality Camel Immune Antibody Libraries (Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, Baptiste Monsion)....Pages 169-187
Construction of Chicken Antibody Libraries (Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt)....Pages 189-203
Construction and Selection of Affilin® Phage Display Libraries (Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, Eva Bosse-Doenecke)....Pages 205-238
Construction of a Synthetic Antibody Gene Library for the Selection of Intrabodies and Antibodies (Déborah Caucheteur, Gautier Robin, Vincent Parez, Pierre Martineau)....Pages 239-253
Targeting Intracellular Antigens with pMHC-Binding Antibodies: A Phage Display Approach (Zhihao Wu, Brian H. Santich, Hong Liu, Cheng Liu, Nai-Kong V. Cheung)....Pages 255-269
Front Matter ....Pages 271-271
Parallelized Antibody Selection in Microtiter Plates (Giulio Russo, Doris Meier, Saskia Helmsing, Esther Wenzel, Fabian Oberle, André Frenzel et al.)....Pages 273-284
Mass Spectrometry Immuno Assay (MSIA™) Streptavidin Disposable Automation Research Tips (D.A.R.T’s®) Antibody Phage Display Biopanning (Chai Fung Chin, Yee Siew Choong, Theam Soon Lim)....Pages 285-299
Magnetic Nanoparticle-Based Semi-Automated Panning for High-Throughput Antibody Selection (Angela Chiew Wen Ch’ng, Nurul Hamizah Binti Hamidon, Zoltán Konthur, Theam Soon Lim)....Pages 301-319
Phage Display and Selections on Cells (Wieland Fahr, André Frenzel)....Pages 321-330
Combine Phage Antibody Display Library Selection on Patient Tissue Specimens with Laser Capture Microdissection to Identify Novel Human Antibodies Targeting Clinically Relevant Tumor Antigens (Yang Su, Scott Bidlingmaier, Nam-Kyung Lee, Bin Liu)....Pages 331-347
Antibody Isolation From a Human Synthetic Combinatorial and Other Libraries of Single-Chain Antibodies (Almog Bitton, Limor Nahary, Itai Benhar)....Pages 349-363
Screening Phage-Display Antibody Libraries Using Protein Arrays (Ricardo Jara-Acevedo, Paula Díez, María González-González, Rosa María Dégano, Nieves Ibarrola, Rafael Góngora et al.)....Pages 365-380
Antibody Selection on FFPE Tissue Slides (Andre ten Haaf, Stefan Gattenlöhner, Mehmet Kemal Tur)....Pages 381-391
Antibody Affinity and Stability Maturation by Error-Prone PCR (Tobias Unkauf, Michael Hust, André Frenzel)....Pages 393-407
Front Matter ....Pages 409-409
Upgrading Affinity Screening Experiments by Analysis of Next-Generation Sequencing Data (Christian Grohmann, Michael Blank)....Pages 411-424
Next-Generation DNA Sequencing of VH/VL Repertoires: A Primer and Guide to Applications in Single-Domain Antibody Discovery (Kevin A. Henry)....Pages 425-446
High-Throughput IgG Reformatting and Expression (Chao-Guang Chen, Georgina Sansome, Michael J. Wilson, Con Panousis)....Pages 447-461
Monitoring Phage Biopanning by Next-Generation Sequencing (Anna Vaisman-Mentesh, Yariv Wine)....Pages 463-473
Front Matter ....Pages 475-475
ORFeome Phage Display (Jonas Zantow, Gustavo Marçal Schmidt Garcia Moreira, Stefan Dübel, Michael Hust)....Pages 477-495
Epitope Mapping by Phage Display (Gustavo Marçal Schmidt Garcia Moreira, Viola Fühner, Michael Hust)....Pages 497-518
Metasecretome Phage Display (Milica Ciric, Filomena Ng, Jasna Rakonjac, Dragana Gagic)....Pages 519-534
Phagekines: Screening Binding Properties and Biological Activity of Functional Cytokines Displayed on Phages (Gertrudis Rojas, Tania Carmenate)....Pages 535-560
Back Matter ....Pages 561-562

✦ Subjects

Immunology