pH is an intracellular effector controlling differentiation of oligodendrocyte precursors in culture via activation of the ERK1/2 pathway
✍ Scribed by Frédéric Bernard; Peter Vanhoutte; Amar Bennasroune; Gérard Labourdette; Martine Perraut; Dominique Aunis; Stéphane Gaillard
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 336 KB
- Volume
- 84
- Category
- Article
- ISSN
- 0360-4012
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✦ Synopsis
Abstract
We reported previously that onset of oligodendrocyte precursor cell (OPC) differentiation is accompanied by an increase in intracellular pH (pH~i~). We show that OPC differentiation is dependent primarily on a permissive pH~i~ value. The highest differentiation levels were observed for pH~i~ values around 7.15 and inhibition of differentiation was observed at slightly more acidic or alkaline values. Clamping the pH~i~ of OPCs at 7.15 caused a transient activation of ERK1/2 that was not observed at more acidic or alkaline values. Furthermore, inhibition of ERK activation with the UO126 compound totally prevented OPC differentiation in response to pH~i~ shift. These results indicate that pH~i~, acting through the ERK1/2 pathway, is a key determinant for oligodendrocyte differentiation. We also show that this pH~i~ pathway is involved in the process of retinoic acid‐induced OPC differentiation. © 2006 Wiley‐Liss, Inc.