pH-dependent secretion of procainamide into saliva

✍ Scribed by Jeffrey R. Koup; William J. Jusko; Allen L. Goldfarb

• Book ID: 102409535
• Publisher: John Wiley and Sons
• Year: 1975
• Tongue: English
• Weight: 314 KB
• Volume: 64
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600641226

✦ Synopsis

occur with human or animal blood would not be expected to have any effect, no buffering was used. Urine samples, however, vary significantly in pH and need pH adjustment to 6.8.

The character of the fluorescent spectrum changed as the plate dried. The peak became larger and more defined a t the given excitation and emission wavelengths (362 and 422 nm, respectively). There was also a significant shift of both the excitation and emission maximum wavelengths to 330 and 480 nm, respectively, when tribromsalan was in 7.5% acetic acid in methanol. Spectra obtained with wet plates resembled those in 7.5% acetic acid in methanol. The change of fluorescence in the presence of acid was also observed by other investigators with aspirin (9). Drying the plate for more than 24 hr in the air provided peaks with consistent intensities without a shift of maximum wavelengths on the fluorescent spectrophotometer.