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PGRP-SB1: An N-acetylmuramoyl l-alanine amidase with antibacterial activity

✍ Scribed by Peter Mellroth; Håkan Steiner


Book ID
116293755
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
199 KB
Volume
350
Category
Article
ISSN
0006-291X

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N-acetylmuramoyl-l-alanine amidase assay
✍ Philippe Margot; Claude-Alain H. Roten; Dimitri Karamata 📂 Article 📅 1991 🏛 Elsevier Science 🌐 English ⚖ 468 KB

A sensitive and highly reproducible assay for N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28) was devised, based on specific and homogeneous L-[14C]alanine labeling of the substrate, the peptidoglycan. The method involves partial purification of both the enzyme and the substrate and monitoring the