๐”– Bobbio Scriptorium
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pGreen: a versatile and flexible binary Ti vector for Agrobacterium-mediated plant transformation

โœ Scribed by Roger P. Hellens; E. Anne Edwards; Nicola R. Leyland; Samantha Bean; Philip M. Mullineaux


Book ID
110236073
Publisher
Springer
Year
2000
Tongue
English
Weight
171 KB
Volume
42
Category
Article
ISSN
0167-4412

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โœ Teresa Mozo; Paul J. J. Hooykaas ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Springer ๐ŸŒ English โš– 852 KB

The loxP-Cre site-specific recombination system of phage P1 was used to develop a novel strategy to construct cointegrate vectors for Agrobacterium-mediated plant transformation. A pTi disarmed helper plasmid (pAL1166) was constructed by replacing the oncogenic T-DNA by a loxP sequence and a spectin