PGF2α increases FGF-2 and FGFR2 trafficking in Py1a rat osteoblasts via clathrin independent and importin β dependent pathway
✍ Scribed by Luigi Marchetti; Maria G. Sabbieti; Dimitrios Agas; Maura Menghi; Giovanni Materazzi; Giovanna Menghi; Marja M. Hurley
- Publisher
- John Wiley and Sons
- Year
- 2006
- Tongue
- English
- Weight
- 869 KB
- Volume
- 97
- Category
- Article
- ISSN
- 0730-2312
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✦ Synopsis
Abstract
Previous studies showed that prostaglandin F~2α~ (PGF~2α~) stimulated fibroblast growth factor‐2 (FGF‐2) and fibroblast growth factor receptor 2 (FGFR2) cytosolic and nuclear accumulation, however, the endocytic pathway has not been elucidated. This study demonstrates that although PGF~2α~ increased the formation of clathrin‐coated structures in Py1a rat osteoblasts, they were not involved in FGF‐2 and FGFR2 trafficking. PGF~2α~ increased binding of FGF‐2 and FGFR2 and co‐localization of reactive sites in addition to nuclear translocation at the nuclear pore complex level. FGF‐2 and FGFR2 were in close spatial correlation with importin β, further supporting nuclear import of the FGF‐2/FGFR2 complex. Immunogold and immunofluorescence techniques as well as Western blotting demonstrated increased importin β protein labeling in response to PGF~2α~. Similar to PGF~2α~, phorbol 12‐myristate 13‐acetate (PMA) also increased importin β protein. These data strongly suggest that prostaglandins may regulate osteoblast metabolism via FGF‐2/FGFR2/importin β nuclear trafficking. J. Cell. Biochem. 97: 1379–1392, 2006. © 2005 Wiley‐Liss, Inc.