Petite-negative mutants of Saccharomyces cerevisiae
β Scribed by Lancashire, William E. ;Payton, Mark A. ;Webber, Martin J. ;Hartley, Brian S.
- Publisher
- Springer
- Year
- 1981
- Tongue
- English
- Weight
- 167 KB
- Volume
- 181
- Category
- Article
- ISSN
- 0026-8925
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β¦ Synopsis
A series of yeast mutants has been isolated with the inability to grow on fermentable carbon sources whilst growing normaily on ethanol media. One of the mutants, namely MC16/ 206 lacks pyruvate decarboxylase activity and does not grow on glucose at 37Β°C but grows on both ethanol and glucose at 27 Β° C. In this strain rhopetites are non-viable.
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Mutations in two genes (cdc8 and cdc21) required for nuclear and mitochondrial DNA synthesis in Saccharomyces cerevisiae result in a 6- to 11-fold increase in the rate of mitotic segregation of petites at the permissive temperature. The defect in DNA replication and the increased rate of petite prod