✦ LIBER ✦

Petite mutants of sophorolipid-producing Candida yeasts

✍ Scribed by Dr. Rolf K. Hommel; Annette Baum; Hans-Peter Kleber

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 244 KB
Volume: 34
Category: Article
ISSN: 0233-111X
DOI: 10.1002/jobm.3620340112

No coin nor oath required. For personal study only.

✦ Synopsis

Treatment of both Cniididu npicoln IMET 43747 and Candid0 hoinhicolu ATCC 22214 with N-methyl-N'-nitro-N-nitroso guanidine resulted in formation of auxotrophic mutants and cytochrome ovidase negative mutants. The deficiency of intact cytochrome oxidase did not affect the ability to produce sophorose lipid. This indicates that intact mitochondria1 energy supplying system is not necessary for the production of the extracellular sophorose lipids by both Cuiididrr yeasts.

Among a variety of yeasts some Caizdidu species are able to produce and excrete large amounts of sophorose lipids when growing on different carbon sources (SPENCER et al. 1979). These lipids consists of hydroxylated fatty acids glycosides of partially acylated sophorose. The regulation of the biosynthesis seems to be strongly connected with the carbohydrate metabolism (HOMMEL and HUSE 1993). Recently, we reported the cyanideresistant respiration of stationary cells of the obligate aerobic C. rryicola (HOMMEL et (11. 1994). However, the sophorose lipid biosynthesis is an energy consuming process as discussed by LINTON (1991). Therefore, we studied both C. upicola and C. hombicolu in respect of respiratory deficient mutants. The present paper presents preliminary results which show that petite mutants from both Cundida strains can be induced and that these mutants were not restricted in their ability to produce sophorose lipid.

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