Pet-1, a novel ETS domain factor that can activate neuronal nAchR gene transcription
✍ Scribed by Fyodorov, Dmitry ;Nelson, Tom ;Deneris, Evan
- Publisher
- John Wiley and Sons
- Year
- 1998
- Tongue
- English
- Weight
- 322 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0022-3034
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✦ Synopsis
We report a cDNA clone prepared recently identified a neural cell-type specific enhancer, from adrenal chromaffin-derived PC12 cell RNA that b43, within the 3-untranslated exon of the neuronal encodes a novel ETS-domain factor, Pet-1. The denicotinic acetylcholine receptor (nAchR) b4 subunit duced primary structure of Pet-1 is composed of 340 gene. Similar to Pet-1, the b4 gene is also expressed amino acids and the encoded polypeptide has a prein PC12 cells. The presence of putative ETS-domain dicted molecular mass of 35.4 kD. The pattern of Petbinding sites in the b43 enhancer led us to hypothe-1 gene expression in the neonatal rat is highly resize that members of the ets gene family activate neustricted and suggests that Pet-1 functions primarily in ronal nAchR genes. Cotransfection assays show that the nervous system. Adrenal gland expresses the high-Pet-1 can activate reporter gene transcription in a est level of Pet-1 among the tissues examined. In situ b43 enhancer-dependent and cell type-dependent hybridization indicates that Pet-1 is expressed in the manner. Our results lead us to hypothesize that Petadrenal medulla but not the adrenal cortex. Slightly 1 acts as a transcriptional regulator of downstream weaker Pet-1 hybridization is detected in brain and target genes involved in cholinergic neurotransmislow levels are detectable in intestine and eye. Pet-1 sion.