Development of pronuclei from human sper
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Ohsumi, Keita ;Katagiri, Chiaki ;Yanagimachi, Ryuzo
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Article
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1986
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John Wiley and Sons
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English
β 736 KB
Human spermatozoa were demembranated with Triton X-100 (TX) and injected into the mature eggs of Xenopus lueuis. The nuclei of these spermatozoa decondensed and developed into pronuclei. Chromosomes did not appear in the eggs until the end of a 5-hr incubation period. When the demembranated human sp