✦ LIBER ✦

Peroxisome proliferation, adipocyte determination and differentiation of C3H10T1/2 fibroblast cells induced by humic acid: Induction of PPAR in diverse cells

✍ Scribed by Yashang Lee; Tien-Shang Huang; Mei-Ling Yang; Lan-Ru Huang; Ching-Hsein Chen; Fung-Jou Lu

Publisher: John Wiley and Sons
Year: 1999
Tongue: English
Weight: 187 KB
Volume: 179
Category: Article
ISSN: 0021-9541
DOI: 10.1002/(sici)1097-4652(199905)179:2<218::aid-jcp12>3.0.co;2-u

No coin nor oath required. For personal study only.

✦ Synopsis

Humic acid, a high-molecular-weight polyphenolic compound, exists abundantly in soil, natural water, and various terrestrial and aquatic environments. Humic acid causes peroxisome proliferation in mouse liver and induces the expression of peroxisome proliferator activated receptor (PPAR) in BNL CL.2 cells. Both cytotoxicity and flow cytometry show that humic acid inhibits the growth of C3H10T1/2 cells at G 1 phase. C3H10T1/2 fibroblast cells express PPAR␥ and the adipocyte P2 (aP2) genes which convert into adipocytes after being treated with humic acid. Our findings may provide a unique model for studying the molecular control of determination and differentiation of mesodermal cell lineages.

📜 SIMILAR VOLUMES

Perturbation of EGF-activated MEK1 and P

Perturbation of EGF-activated MEK1 and PKB signal pathways by TGF-β1 correlates with perturbation of EGF-induced cyclin D1 and DNA synthesis by TGF-β1 in C3H 10T1/2 cells

✍ Shaochun Yan; Shelly Krebs; Kirk J. Leister; Charles E. Wenner 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 302 KB 👁 1 views

In mouse C3H 10T1/2 cells, we previously reported that TGF-beta1 first delays and later potentiates EGF-induced DNA synthesis corresponding to an inhibition of EGF-induced cyclin D1 expression at t = 13 h. We report here that in accord with DNA synthesis kinetics, TGF-beta1 initially suppresses EGF-