Peroxidase–ferrocene modified carbon paste electrode as an amperometric sensor for the hydrogen peroxide assay

✍ Scribed by Pilar Domínguez Sánchez; Arturo José Miranda Ordieres; Agustín Costa García; Paulino Tuñón Blanco

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 377 KB
• Volume: 3
• Category: Article
• ISSN: 1040-0397
• DOI: 10.1002/elan.1140030407

✦ Synopsis

A new enzymatic amperometric sensor for hydrogen peroxide based on a modified carbon paste electrode is described. Horseradish peroxidase and ferrocene mediator were immobilized by dissolution or dispersion in a graphite-paraffin oil paste. To prevent losses of immobilized chemicals under operational conditions, the electrode surface was coated with a Nafion membrane. The device exhibits a relatively fast response time (42 s for 95% of the steady-state response) and yields currents linearly related to the hydrogen peroxide concentration over the lo-' to mol/L range in both static and flow injection operations. The slope of the calibration plot remained constant for more than 20 days. Flow injection assays of hydrogen peroxide at a sampling rate of about 70 injections per hour, with a relative standard deviation of 1.4%, are possible. An easy and low cost preparation procedure is described.