Permeabilization of microorganisms by Tr
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G.F. Miozzari; P. Niederberger; R. Hu¨tter
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Article
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1978
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Elsevier Science
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English
β 904 KB
A simple permeabilization procedure has been developed which allows the reliable determination of enzyme activities in situ in a variety of different microorganisms. Permeabilization is obtained by freezing cell suspensions in the presence of a low concentration of the anionic detergent Triton X-100