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Permeabilization of Yarrowia lipolytica cells by triton X-100

✍ Scribed by D. Galabova; B. Tuleva; D. Spasova


Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
622 KB
Volume
18
Category
Article
ISSN
0141-0229

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A simple permeabilization procedure has been developed which allows the reliable determination of enzyme activities in situ in a variety of different microorganisms. Permeabilization is obtained by freezing cell suspensions in the presence of a low concentration of the anionic detergent Triton X-100