Permanent cannula-injection system for intracerebral injections in small animals

✍ Scribed by Lee R. Grunden; Geoffrey E. Linburn

• Publisher: John Wiley and Sons
• Year: 1969
• Tongue: English
• Weight: 351 KB
• Volume: 58
• Category: Article
• ISSN: 0022-3549
• DOI: 10.1002/jps.2600580927

✦ Synopsis

The procedure for constructing a simplified cannulainjection system for making repeated intracerebral injections in conscious, free-moving animals is described. The system is made from standard hypodermic needles and polyethylene tubing. The absence of screw joints and the small size make this system particularly useful for chronic injections in small animals. Additional advantages are discussed.

Keyphrases 0 Cannula-injection system-erebral injections 0 Intracerebral injections-small animals 0 Brain-localized injection 17 Intraventricular injections-rats, chickens 0 Diagramcannula-injection system Much interest has developed in recent years in the chemistry and pharmacology of the brain. A useful tool for studies in the neurological sciences has been the implanted cannula (1). Implanted with a stereotaxic apparatus for precise localization in a specific area of the brain, the cannula permits the introduction of a given amount of compound directly into the brain of an unanesthetized animal. The in uivo effects of the compound may then be studied with a verifiable knowledge of the specific area treated.

Few detailed descriptions (2-5) of a chronic cannulainjection system suitable for small animals are available. The cannula-injection system which has been developed and described in detail here has several simplifying features which make it compact and easy to construct and use. Moreover, the cannula is easily reclaimed for repeated implants.