Periplasmic superoxide dismutases inAquaspirillum magnetotacticum

✍ Scribed by K. A. Short; R. P. Blakemore

• Book ID: 104770072
• Publisher: Springer
• Year: 1989
• Tongue: English
• Weight: 697 KB
• Volume: 152
• Category: Article
• ISSN: 0302-8933
• DOI: 10.1007/bf00425171

✦ Synopsis

Aquaspirillum magnetotacticum MS-1 cells cultured microaerobically (dissolved O2 tension 1% of saturation), expressed proteins with superoxide dismutase (SOD) activity. The majority (roughly 95%) of total cell superoxide dismutase activity was located in the cell periplasm with little or no activity in the cell cytoplasm. Irontype SOD (FeSOD) contributed 88% of the total activity activity detected, although a manganese-type SOD (MnSOD) was present in the periplasm as well. Cells cultured at a higher dissolved Oa tension (10% of saturation) expressed increased activity of the MnSOD relative to that of the FeSOD.