✦ LIBER ✦

Peripheral-blood mononuclear cell responses to recombinant hepatitis C virus antigens in patients with chronic hepatitis C

✍ Scribed by Haim Schupper; Paul Hayashi; James Scheffel; Sherri Acettuno; Teresa Paglieroni; Paul V. Holland; Jerome B. Zeldis

Publisher: John Wiley and Sons
Year: 1993
Tongue: English
Weight: 614 KB
Volume: 18
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840180507

No coin nor oath required. For personal study only.

✦ Synopsis

Peripheral blood mononuclear cell proliferative responses in uitro to recombinant yeast or Escherichia coli hepatitis C virus fusion proteins were evaluated in 20 patients with chronic hepatitis C who were reactive for antibody to hepatitis C virus (on enzyme immunoassay, version 2.0, and a four-antigen recombinant immunoblot assay). Twenty age-matched, healthy individuals negative for antibody to hepatitis C virus were used as a control group. Peripheral-blood mononuclear cells from all chronic hepatitis C patients with antibodies to hepatitis C virus antigens c22 and c100-3 proliferated in uitro in response to the corresponding recombinant hepatitis C virus fusion protein. Peripheral-blood mononuclear cells from 75% of patients infected with hepatitis C virus proliferated in response to cytidine monophosphate-keto-3-deoxyoctulosonic acid-core recombinant antigen but there was no proliferative response to cytidine monophosphate-keto-3deoxyoctulosonic acid-EF (derived from the NS5 region). All hepatitis C virus-infected patients had 33c antibody, but peripheral-blood mononuclear cells from only 9 of 14 (64%) proliferated in uitro in response to 33c. Ninety-five percent of all hepatitis C virus-infected patients had peripheral-blood mononuclear cells that proliferated in response to at least one recombinant hepatitis C virus fusion protein. The numbers and percentages of CD3 T cells, CD19 B cells and natural killer cells from patients with chronic hepatitis C virus infection did not differ from those in the healthy control group. However, the number of non-msljor histocompatibility complex-restricted cytotoxic T cells (CD3-positive, CD56-positive, CD16positive) was increased in patients with chronic hepatitis C virus infection (p < 0.05).

📜 SIMILAR VOLUMES

Quantitation of hepatitis C virus in liv

Quantitation of hepatitis C virus in liver and peripheral blood mononuclear cells from patients with chronic hepatitis C virus infection

✍ Martín, Julio; Navas, Sonia; Quiroga, Juan Antonio; Colucci, Giuseppe; Pardo, Ma 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 90 KB 👁 2 views

Since the natural history of hepatitis C virusassociated liver disease and the therapeutic responsiveness might vary according to liver and blood mononuclear cells viral levels, it may be important to quantitate viral RNA in liver, blood mononuclear cells and serum, and to compare these data with ge

Hepatitis C virus RNA in peripheral bloo

Hepatitis C virus RNA in peripheral blood mononuclear cells: Comparing acute and chronic hepatitis C virus infection

✍ T Chang; K Young; Y Yang; H Lei; H Wu 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 174 KB 👁 2 views

Hepatitis C virus (HCV) can infect peripheral blood mately 10 kb. 2 Acute posttransfusion hepatitis C mononuclear cells (PBMC) of patients with chronic HCV (PTHC) is followed by chronic hepatitis in about 50% infection. No data are available on PBMC testing for of the cases. Chronic hepatitis may al

Fluorescent ?in situ? hybridization of h

Fluorescent ?in situ? hybridization of hepatitis C virus RNA in peripheral blood mononuclear cells from patients with chronic hepatitis C

✍ Rodr�guez-I�igo, Elena; Casqueiro, Mercedes; Navas, Sonia; Bartolom�, Javier; Pa 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 166 KB 👁 2 views

Although the liver is the main target for hepatitis C virus (HCV) infection, HCV RNA of positive and negative polarity has also been detected in peripheral blood mononuclear cells (PBMCs) by polymerase chain reaction. However, no data have been published on the relationship between the number of HCV

Hepatitis C virus RNA in peripheral bloo

Hepatitis C virus RNA in peripheral blood mononuclear cells: Relation with response to interferon treatment

✍ Dr. Gloria Taliani; Maria Coneetta Badolato; Rosina Lecce; Giuseppina Poliandri; 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 634 KB

## Abstract The polymerase chain reaction (PCR) was used to investigate the presence of positive and negative hepatitis C virus (HCV) RNA strands in serumand peripheral blood mononuclear cells (PBMC) of 20 patients with histologically proven HCV‐ related chronic liver disease. All patients complete

Hepatitis C virus-RNAs in plasma and in

Hepatitis C virus-RNAs in plasma and in peripheral blood mononuclear cells of hemophiliacs with chronic hepatitis C: Evidence for viral replication in peripheral blood mononuclear cells

✍ M. Willems; K. Peerlinck; H. Moshage; I. Deleu; C. Van Den Eynde; J. Vermylen; D 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 788 KB

## Abstract Hemophiliacs who have been exposed to unbeaten and/or dry heated pooled clotting factor concentrates are at high risk of chronic hepatitis C. Although the mechanism and site of hepatitis C virus (HCV) replication are not yet known, HCV is thought to replicate through a complementary neg

T- and B-cell responses to different hep

T- and B-cell responses to different hepatitis C virus antigens in patients with chronic hepatitis C infection and in healthy anti- hepatitis C virus—positive blood donors without viremi

✍ M Lechmann; H G Ihlenfeldt; I Braunschweiger; G Giers; G Jung; B Matz; R Kaiser; 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 244 KB 👁 2 views

¨b-37 months [range, 28 months-23 years] as documented by the first ingen, Tu ¨bingen, Germany.