Perinatal Stem Cells

✍ Scribed by Curtis L. Cetrulo Jr., Kyle Cetrulo

• Publisher: Wiley-Blackwell
• Year: 2009
• Tongue: English
• Leaves: 243
• Edition: 1
• Category: Library

✦ Synopsis

Discover how perinatal stem cells may prove to be a more powerful therapeutic tool than embryonic stem cellsNormally discarded as medical waste, perinatal stem cells are an incredible source of stem cells and are gaining phenomenal research attention for their potential to become a more powerful alternative to the controversial embryonic stem cells. In response to this growing field, this book provides a thorough tutorial on the current state of the art in perinatal stem cells.Presented by a panel of authorities whose expertise reaches from research to hematology to tissue engineering and beyond, this important volume covers all of the sources of stem cells found throughout pregnancy, revealing the underlying biology and potential therapeutic uses of each:Post-gestational maternal peripheral bloodUmbilical cord bloodWharton's jellyAmniotic fluidAmnion lining the amniotic cavityUmbilical veinChorionic mesenchymal stromal cellsMulti-potent cellsPlacenta stem cellsThis book is a bountiful source for researchers, graduate students, cell biologists, gene therapists, obstetricians and gynecologists, and professionals engaged in regenerative medicine and developmental biology.

✦ Table of Contents

PERINATAL STEM CELLS......Page 4
CONTENTS......Page 8
PREFACE......Page 14
CONTRIBUTORS......Page 16
INTRODUCTION: PERINATAL STEM CELLS......Page 18
Hematopoietic Stem Cell Transplants: Adult Donor Collection......Page 22
Hematopoietic Stem Cell Transplants: Adult Donor Testing......Page 23
Hematopoietic Stem Cell Transplants: Recipient Issues......Page 24
Collection and Processing of Cord Blood Units......Page 25
Bone Marrow versus Single Cord Blood: Pediatric......Page 26
Bone Marrow versus Cord Blood: Adults......Page 27
Cord Blood Transplant: Advantages and Disadvantages......Page 28
Double Cord Blood Transplants: Ablative Regimens......Page 29
Double Cord Blood Transplant: Non-Myeloablative Regimens......Page 30
Chimerism......Page 31
Predicting the Winner......Page 33
Are Two Cords Better Than One?......Page 34
References......Page 35
Introduction: Hematopoietic Stem Cells, the Therapeutic Distributed Stem Cells in Umbilical Cord Blood......Page 42
Biological Barriers to Hematopoietic Stem Cell Therapy......Page 45
Umbilical Cord Blood: An Ideal Source of Therapeutic Hematopoietic Stem Cells......Page 48
Shortcomings of Ex Vivo HSC Expansion Strategies......Page 50
A Novel Strategy With Potential to Enable Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells......Page 53
Acknowledgments......Page 55
References......Page 56
Introduction......Page 62
Principles of Tissue Engineering......Page 63
Examples of Possible Use and Implications of Perinatal Stem Cells......Page 64
Amniotic Derived Stem Cells (hAMSC/Placental!)......Page 65
Amniotic Fluid-Derived Stem Cells (Nonplacental!)......Page 66
Conclusion......Page 67
References......Page 69
The Fetus as Recipient for Pre- and Perinatal Stem Cell Transplantation......Page 72
Prenatal Cell Transplantation: Current Experience......Page 73
How Do We Improve the Success of Prenatal Stem Cell Transplantation?......Page 74
Fetal Gene Therapy as a New Strategy......Page 75
Current Issues of Postnatal Gene Therapy......Page 76
Prenatal Gene Therapy......Page 78
Fetal Gene Therapy Targeting Hematopoietic Stem Cells......Page 79
Safety Aspects of Prenatal Gene Therapy......Page 80
References......Page 81
Preparation and Culture of UC–MSC......Page 90
Characteristics–Properties and Bone Marrow Comparison......Page 92
UC–MSC as Feeder Layer for Expansion of Human Cells......Page 94
References......Page 96
Mesenchymal Stromal Cells......Page 100
Defining Primitive Stromal Cells: Differences between Fetal and Adult MSCs......Page 102
Umbilical Cord MSCs......Page 103
Other MSC-Like Cells from Umbilical Cord......Page 104
Comparison of WJCs to Adult Derived MSCs......Page 105
In Vitro Differentiation of WJCs......Page 106
Transplantation of WJCs......Page 107
WJCs as Primitive Stromal Cells......Page 110
References......Page 111
The Role of Vascular Stem Cells in Postnatal Blood Vessel Formation: Endothelial Progenitor Cells and Postnatal Vasculogenesis......Page 116
EPCs, Vasculogenesis and Angiogenesis......Page 117
EPCs and Tissue Engineering......Page 118
EPCs and Cardiovascular Tissue Engineering......Page 119
References......Page 120
Introduction......Page 124
Mast Cell Triggers......Page 127
Mast Cell Mediators......Page 130
Selective Release of Mast Cell Mediators......Page 132
Natural Mast Cell Secretion Inhibitors......Page 133
Inflammatory Skin Diseases......Page 134
Fibromyalgia Syndrome......Page 136
Asthma......Page 138
Inflammatory Arthritis......Page 140
Coronary Inflammation......Page 141
Conclusion......Page 143
References......Page 144
Amniotic Fluid Cells......Page 168
The Discovery of Stem Cells in Amniotic Fluid......Page 170
Characteristics of Amniotic Fluid Derived Stem Cells......Page 171
Amniotic Fluid as a Promising Alternative Source for Neural Stem Cells......Page 174
References......Page 176
Introduction......Page 180
Isolation and Culture of hAE Cells......Page 181
Stem Cell Characteristics of Amniotic Epithelium-Derived Cells......Page 182
Differentiation of hAE Cells......Page 183
Future Directions......Page 184
Conclusions......Page 185
References......Page 186
Human Umbilical Vein Endothelial Cells......Page 190
Role of Angiogenesis in the Pathophysiology of Disease......Page 191
Studies of the Effects of Statins on Angiogenesis Using Both In Vivo Models and In Vitro HUVEC and HDMEC Models for Angiogenesis......Page 192
References......Page 206
The Hematopoietic System......Page 210
Historical Perspective on Placental Hematopoiesis......Page 211
Development and Structure of the Mouse Placenta......Page 213
Hematopoietic Activity in the Mouse Placenta......Page 214
Phenotype of Placental HSCs......Page 215
Origin and Localization of Placental HSCs......Page 216
Summary and Future Directions......Page 219
References......Page 220
The Placenta is a Source of Stem Cells......Page 224
Transfer of Fetal Progenitor Cells During Gestation......Page 225
Fetal Progenitor Cells in Maternal Tissues......Page 226
Future Therapies......Page 227
References......Page 228
INDEX......Page 230