Performance of integrated glucose and lactate thin-film microbiosensors for clinical analysers

✍ Scribed by G. Urban; G. Jobst; E. Aschauer; O. Tilado; P. Svasek; M. Varahram; Ch. Ritter; J. Riegebauer

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 457 KB
• Volume: 19
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/0925-4005(93)01095-l

✦ Synopsis

Integrated thin-film glucose and lactate sensors were produced using thin photo-pattemable multi-enzyme membranes. The glucose and lactate sensors are based on the measurement of H,O* on a Pt electrode produced by the enzymes glucose oxidase (GOD) and lactate oxidasc (LOD), respectively, which were entrapped in photopatterned pHEMA hydrogel membranes. To prevent electrochemical interferences and fouling of the Pt electrode an electropolymerized semipermeable membrane was used. A second enzyme membrane layer containing the enzyme catalase was immobilized above the oxidase enzymes. To separate the hvo enzyme layers and to introduce an additional diffusion barrier a third pHEMA membrane without enzyme was placed between the oxidase and catalase enzyme membranes. The intended use of this device is the measurement of undiluted biological fluids in a clinical analyser. The sensors were tested in buffer solutions and undiluted serum and exhibited excellent performances.