Performance of a highly sensitive optical waveguide Mach-Zehnder interferometer immunosensor

✍ Scribed by R.G. Heideman; R.P.H. Kooyman; J. Greve

• Book ID: 103960898
• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 525 KB
• Volume: 10
• Category: Article
• ISSN: 0925-4005
• DOI: 10.1016/0925-4005(93)87008-d

✦ Synopsis

We describe a highly sensitive sensor which uses the evanescent field of a reusable planar optical waveguide as the sensing element . The waveguide used is optimized to obtain a steep dependence of the propagation velocity on the refractive-index profile near the surface, The adsorption of a layer of proteins thus results in a phase change, which is measured interferometrically using a Mach-Zehnder interferometer set-up . The stability of the interferometer is such that phase changes z (I x 10 -')2st per hour can be measured, Immunoreactions have been monitored down to concentrations of 5 x 10 -I' M of a 40 kDa protein .