Performance of a Glucose/O2 Enzymatic Biofuel Cell Containing a Mediated Melanocarpus albomyces Laccase Cathode in a Physiological Buffer
✍ Scribed by P. Kavanagh; S. Boland; P. Jenkins; D. Leech
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 267 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1615-6846
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✦ Synopsis
Abstract
We report on the performance of a prototype glucose/O~2~ biofuel cell in a physiological buffer. The cell consists of cathode based on Melanocarpus albomyces laccase (with a reported T1 copper redox potential of + 0.26 V vs. Ag/AgCl) co‐immobilised with an osmium redox polymer mediating film on glassy carbon (GC) separated by a Nafion 117 membrane from a GC anode in anolyte of glucose oxidase, N,N,N′,N′‐tetramethyl‐p‐phenylenediamine (TMPD) as a mediator, all immersed in pH 7.4 phosphate buffer solution containing 0.15 M NaCl and dissolved oxygen, thermostated at 37 °C, mimicking physiological conditions. Two osmium redox polymers are employed for cathodic mediation: [Os(2,2′‐bipyridine)~2~(polyvinylimidazole)~10~Cl]^+/2+^ (E°′ 0.22 V vs. Ag/AgCl) and [Os(4,4′‐dichloro‐2,2′‐bipyridine)~2~(polyvinylimidazole)~10~Cl]^+/2+^ (E°′ 0.35 V vs. Ag/AgCl). Power outputs of 52 μW cm^–2^ at 0.21 V and 17 μW cm^–2^ at 0.34 V were obtained for assembled fuel cells containing the respective redox polymer‐mediated laccase cathodes, illustrating the increased power obtained as a result of higher biocatalytic efficiency using a redox polymer with redox potential tailored for the enzyme.