✦ LIBER ✦

Performance evaluation of the FACSCount system: A dedicated system for clinical cellular analysis

✍ Scribed by K. Strauss; I. Hannet; S. Engels; A. Shiba; D. M. Ward; S. Ullery; M. G. Jinguji; J. Valinsky; D. Barnett; A. Orfao; L. Kestens

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 675 KB
Volume: 26
Category: Article
ISSN: 0196-4763
DOI: 10.1002/(sici)1097-0320(19960315)26:1<52::aid-cyto8>3.0.co;2-i

No coin nor oath required. For personal study only.

✦ Synopsis

Flow cytometers are instruments that can determine multiparameter data simultaneously and have a great potential in providing unique information about cells. The FACSCount System is designed as the first dedicated flow cytometer for the clinical laboratory. Its current configuration provides CD4, CD8, and CD3 absolute counts from 100 pI of whole blood. Adapting the FACSCount System to the clinical setting are minimal sample handling, lysis free cell preparation, automated fluorescence gating, built-in calibrated reference beads, and appropriate error code reporting. Quality control checks ensure that reported CD4 counts, important for clinical follow-up and patient management, are accurate, precise, and reproducible across instruments over time. Q 1996 Wiley-Liss, Inc.

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