✦ LIBER ✦
Performance evaluation of an aqueous—organic phase separator for post-column reactions in high-performance liquid chromatography, and its application to the enhanced detection of some basic drugs of abuse
✍ Scribed by I.M. Roy; T.M. Jefferies
- Publisher
- Elsevier Science
- Year
- 1990
- Tongue
- English
- Weight
- 332 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0731-7085
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✦ Synopsis
A phase separator is described that is suitable for post-column HPLC applications. It operates with commonly used HPLC eluents and immiscible organic solvents as long as the two phases remain immiscible. It is compatible with gradient elution systems. Separation efficiency is routinely better than 0.8, which ensures that analyte peak heights are about 95% of the maximum height under these conditions. An application for the detection of pethidine, cocaine, methadone, piritramide and dipipanone at 0.8-1.8 ng on-column loadings is described.